Quantification of Tryptophan and NAD + Proton Magnetization Exchange With Water Using Downfield 1 H MRS in the Human Brain at 7 T
Year: 2025
Type: article
Abstract: ABSTRACT Purpose The purpose of this study was to investigate the effect of magnetization exchange on the measurement of tryptophan and NAD + T 1 relaxation times and to determine the magnetization exchange rates with a two‐spin system model using downfield 1 H MRS spectroscopy at 7 T in human brain. Methods We collected downfield 1 H MRS spectra in the human brain of eight healthy volunteers using a spectrally selective single‐slice sequence (excitation window: 9.7 ± 2 ppm) at 7 T. Alternating selective and broadband saturation recovery experiments were performed to probe magnetization transfer‐dependent changes in T 1 recovery. The apparent T 1 was modeled independently for each experiment for the tryptophan resonance at 10.1 ppm and for the NAD + resonances at 9.3, 9.1, and 8.9 ppm. The magnetization exchange rate was modeled explicitly using a two‐spin model to fit both experiments simultaneously. Results The apparent T 1 relaxation times measured from broadband saturation recovery experiments were significantly longer for each resonance compared to those measured from selective saturation ( p < 0.001). The ratio of broadband to selective T 1 was significantly larger for tryptophan than NAD + (TRP: 19 ± 6 vs. NAD + : 9 ± 3; p < 0.01). Using the two‐spin model, we modeled the T 1 of each resonance (ms): T 1,TRP = 622.3 ± 405.5; T 1,NAD,H2 = 924.9 ± 233.7; T 1,NAD,H6 = 1800.0 ± 985.5; T 1,NAD,H4 = 2057.3 ± 573.5. The chemical exchange rate of tryptophan was 12.3 ± 3.1 Hz; the cross‐relaxation rates of NAD + were (Hz): σ NAD,H2 = 5.7 ± 1.6, σ NAD,H6 = 3.3 ± 0.8, and σ NAD,H4 = 3.0 ± 1.5. The tryptophan exchange rate was significantly faster than the rates for NAD + ( p < 0.001). Conclusion Tryptophan chemical exchange and NAD + cross‐relaxation with water can be quantified in vivo in human brain at 7 T using downfield spectroscopy.
Source: Magnetic Resonance in Medicine
DOI: 10.1002/mrm.70134
Authors: Sophia Swago, Neil E. Wilson, Mark A. Elliott, Ravinder Reddy, Walter R. Witschey, Ravi Prakash Reddy Nanga
Institution: University of Pennsylvania
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,680
Awards: G129147643, G1578487098, G2859360560, Cardiac 1H MRS assessment of NAD+ after Tetralogy of Fallot repair, G4104635947, G7690085217, Novel Imaging Biomarkers of Lipid and Ketone Metabolism in Alzheimer's Disease, Cardiac MR imaging of hemorrhagic reperfusion injury after myocardial infarction
OpenAlex ID: W4415214528