Year: 2025
Type: article
Abstract: Coffee is a widely consumed beverage that has a major impact on economies, markets, and industries; however, the postproduction of coffee drinks results in wasted or spent coffee grounds (SCG). SCG is used as a plant growth medium; however, its suitable level of use is uncertain. This research determined the optimal amount of SCG for root development and its effect on the cells of Allium cepa. The SCG was mixed with sand (S) in different ratios (SCG:S = 40:60, 50:50, 60:40, 70:30, 80:20, 90:10) to evaluate their effects on root growth and cytotoxicity on cells. Root number and length were measured to assess growth, while the mitotic index (MI) and chromosomal abnormalities were analyzed to determine cytotoxic effects. The 40:60 SCG:S mixture produced the highest root growth compared to controls, whereas higher SCG ratios (70:30, 80:20, 90:10) reduced the MI. Various chromosomal abnormalities were observed, such as micronuclei, c-mitosis, fragmented chromosomes, chromosome bridges, sticky chromosomes, and laggard chromosomes. Therefore, the chemical compounds in SCG affected cytotoxicity on A. cepa roots. The most beneficial use of SCG for addition to plant growth media resulted from a precisely defined mixture to balance growth enhancement with potential cytotoxic risk.
Authors: Tanaporn Pengwong, Noppamart Lokkamlue
Institution: Kasetsart University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
Funder: Kasetsart University
OpenAlex ID: W4415230471
FWCI:
Cites:
Cited by:
Related to: