Work

Systematic misclassification of sylvatic dengue virus 2 (DENV-2) infections as “undetermined serotype”: implications in routine RT-qPCR surveillance

Year: 2025

Type: article

Abstract: Recent findings by Suppiah et al. revealed DENV-2 positive samples misclassified as "Undetermined Serotype" when using commercial dengue molecular serotyping assays. This highlights the noninclusion and resulting misclassification of this viral strain as a dengue virus serotype using available molecular tools. Drawing parallels from the notification of the same assay failure against sylvatic DENV-2 strains in Senegal, West Africa, this correspondence advocates for updating the existing tools to account for this viral variant. Development of multiplex assays including both urban and sylvatic strains may enhance dengue virus surveillance and provide real time monitoring of the prevalence of sylvatic viral lineages among recorded dengue infections in surveillance data.

Source: Tropical Medicine and Health

DOI: 10.1186/s41182-025-00827-0

Author: Idrissa Dieng

Institutions: Institut Pasteur de Dakar, Cheikh Anta Diop University

Language: English

Topic: Mosquito-borne diseases and control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,990