Work

Immuno-Golgi method - Integrating immunohistochemistry and Golgi staining to characterize newborn neurons morphology

Year: 2025

Type: article

Abstract: Visualizing neuronal dendritic architecture while simultaneously identifying specific neuronal phenotype is challenging. Here, we present a detailed description of the Immuno-Golgi technique which combines bromodeoxyuridine (BrdU) immunohistochemistry with Golgi impregnation. This straightforward and non-invasive approach enables the three-dimensional visualization and quantitative analysis of dendritic arborization and spine morphology in newborn neurons within the adult rodent brain.

Source: Brain Organoid and Systems Neuroscience Journal

DOI: 10.1016/j.bosn.2025.10.001

Authors: Leandro Rodrigues-Freitas, Luísa Pinto

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,190

Funder: Fundação para a Ciência e a Tecnologia

Award: Sem título