Morphological and Genomic Analysis of Drulisvirus Targeting Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae with K12 and K18 Capsular Types
Year: 2025
Type: article
Abstract: Background: (ESBL-KP) in Indonesia necessitates the exploration of phage therapy as a potential alternative treatment strategy. Materials and Methods: A lytic phage capable of infecting ESBL-KP was isolated from the Cideng River in Jakarta and subsequently characterized morphologically and genomically. Results: A novel lytic phage, designated JKT1, exhibited an icosahedral head (±52 nm diameter), a short tail, and formed large clear plaques with halos on bacterial lawns. JKT1 demonstrated rapid adsorption, a high burst size, and narrow host specificity. JKT1 remained stable at neutral pH and temperatures up to 37°C but lost activity at ≥50°C. Genome analysis revealed a 43,763 bp linear dsDNA genome encoding 57 open reading frames, including a putative depolymerase gene. Phylogenetic and genomic analyses classified JKT1 within the genus Drulisvirus. Conclusions: This study demonstrates JKT1's activity against specific capsular ESBL-KP strains, highlighting its targeted host range and potential for inclusion in phage cocktails for targeting bacterial infections.
Source: PHAGE
Authors: Yulia Rosa Saharman, Yulia Rosa Saharman, Nuridha Audinia Safitri, Yustinus Maladan, Ajeng K. Pramono, Leli Saptawati, Dewi Anggraini, Iftita Rahmatika, Ruby Setiawan, Dodi Safari, Rosantia Sarassari, Rosantia Sarassari, Meity Mardiana
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4415277683