Work

Evaluating the toxicity and efficacy of the endophytic bacterium Kosakonia sp. ZO-Rh4 on antidiabetes and associated complications in a mouse model

Year: 2025

Type: article

Abstract: The endophytic bacterium Kosakonia sp. ZO-Rh4, isolated from ginger, exhibited antioxidant properties in vitro . However, its safety and efficacy have never been assessed in vivo . This study examined the toxicity of the ethyl acetate extract from Kosakonia sp. ZO-Rh4 (KE) in mice and its potential to treat diabetes and its complications. Acute toxicity was investigated with a single KE dosage of 5000 mg/kg body weight (b.w.). Sub-chronic toxicity was studied with a daily dosage of 400 mg/kg b.w. for 45 and 90 days. After 14 days of acute toxicity and 45 or 90 days of daily dosing at 400 mg/kg body weight, all parameters were found to be within normal limits. In vitro antidiabetic tests indicated that KE has a strong inhibitory effect on the α-amylase and α-glucosidase enzymes. An in vivo study was conducted on diabetic mice induced with alloxan monohydrate, measuring the mice's body weight and blood glucose levels weekly. Following the experiment, mouse blood was collected to measure lipid parameters and liver function markers. The liver, kidneys, and pancreatic tissues were evaluated for oxidative stress by measuring malondialdehyde and glutathione levels. KE lowered blood glucose and enhanced body weight depending on the dosage. Furthermore, KE possesses anti -dyslipidemic properties, can restore liver function, and mitigates oxidative stress in all examined organs by decreasing malondialdehyde and increasing glutathione. The study confirmed the relative safety of KE and its effectiveness in treating diabetes and its complications, potentially representing a new option for functional foods and pharmaceuticals. • Kosakonia sp. ZO-Rh4 extract (KE) was obtained from the endophytic Kosakonia sp. ZO-Rh4. • KE was found to be non-toxic in both acute and sub-chronic studies in mice. • KE inhibited α-amylase and α-glucosidase, converting carbohydrates into glucose. • KE had hypoglycemic, antidyslipidemic, and cardiovascular risk-lowering properties. • KE reduced liver damage and oxidative stress in the liver, kidneys, and pancreas.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2025.102319

Authors: Trang Thi Xuan Dai, Tran Chi Linh, Ta Lam Tai

Institutions: Nam Can Tho University, Can Tho University

Language: English

Topic: Natural Antidiabetic Agents Studies

Subfield: Endocrinology, Diabetes and Metabolism

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,700