Picrosirius red, polarized light microscopy, and curvelet transform analysis: An improved method for liver fibrosis assessment
Year: 2025
Type: article
Abstract: Liver fibrosis is a hallmark of chronic liver diseases, characterized by excessive extracellular matrix deposition and structural alterations. Conventional histological assessments rely on qualitative or semi-quantitative scoring systems that may lack sensitivity for subtle fibrotic changes. Here, we present an improved fibrosis evaluation method integrating Picrosirius Red (PSR) staining, polarized light (PL) microscopy, and curvelet transform-based fiber analysis (CT-FIRE). This approach provides a quantitative and reproducible assessment of collagen fiber morphology and composition. Applied to murine models of metabolic dysfunction-associated steatohepatitis (MASH)-induced fibrosis, our method reveals novel insights into fibrosis progression and strain-dependent variations. This enhanced workflow offers a cost-effective, high-resolution tool for fibrosis analysis in preclinical research.
Source: Tissue and Cell
Institutions: Consejo Superior de Investigaciones Científicas, Institut d'Investigacions Biomèdiques de Barcelona, Consorci Institut D'Investigacions Biomediques August Pi I Sunyer, Universitat de Barcelona, Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Biomedical Research Networking Center on Neurodegenerative Diseases
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,680
OpenAlex ID: W4415340724