Exploring the Anti-Inflammatory Potential of Isoembigenin: A Flavonoid Glycoside from Piper aduncum L. — A Case Exploration through In Vitro , In Vivo , and In Silico Models
Year: 2025
Type: article
Abstract: L. has long been used in traditional medicine for its notable antimicrobial, anti-diarrheal, and anti-inflammatory properties. Flavonoids are recognized as the principal bioactive constituents of this plant, among which isoembigenin (IEGN) has been shown to inhibit dendritic cell-mediated inflammatory responses. However, its effects on other immune cell types involved in inflammation remain unclear. In this study, we examined whether IEGN pre-treatment could attenuate macrophage activation triggered by lipopolysaccharide (LPS). Our results demonstrated that IEGN significantly suppressed TNF-α expression in RAW 264.7 macrophages upon LPS stimulation. Moreover, in a murine sepsis model, IEGN pre-treatment markedly reduced the production of proinflammatory cytokines, including TNF-α and IL-1β, shortly after LPS challenge. Importantly, IEGN administration substantially improved the survival rate of BALB/c mice subjected to endotoxin-induced shock. Finally, the molecular docking simulation was a hypothetical assessment of the interaction between IEGN and iNOS and COX-2. Taken together, these findings highlight the therapeutic potential of IEGN as a candidate for preventing inflammation driven by macrophage activation.
Authors: Nguyen Thi Minh Nguyet, Vinh Le Ba, Van Dinh Nguyen, Cuong Nguyen Cao, Huong Dinh Thi, Phong Nguyen Viet, Luyen Bui Thi Thuy, Chang-kyu Lee, So Young Ban, Jong-Tae Park
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: green
Awards: G2448604052, G6431951138
OpenAlex ID: W4415343628