Antibacterial Activity of Nigella sativa Seed Oil Against Oral Pathogens and Its Inhibitory Effects on Acid Production and Biofilm Formation in Streptococcus mutans
Year: 2025
Type: article
Abstract: This study was conducted to investigate the antibacterial activity of Nigella sativa seed oil (HT_01) against several oral pathogens and the inhibitory effects of HT_01 on acid production and biofilm formation in Streptococcus mutans. The antibacterial activity of HT_01 was tested using the disk diffusion and microdilution methods according to Clinical & Laboratory Standards Institute and Korean Ministry of Food and Drug Safety guidelines. The acid-producing ability test and biofilm formation ability were performed according to Korean Ministry of Food and Drug Safety guidelines. The inhibition zone around the disks containing 50 μL of 10% HT_01 were 42.5 ± 2.5 mm for S. mutans, 41.4 ± 4 mm for Porphyromonas gingivalis, 45 ± 3 mm for Prevotella intermedia, and 28 ± 3 mm for Fusobacterium nucleatum, indicating a strong antibacterial effect. Analysis of acid production showed that the pH of culture broth of S. mutans was 4.23 ± 0.05, whereas culture broth containing of 0.04% and 0.05% HT_01 had neutral pH values of 7.35 ± 0.02 and 7.21 ± 0.04, respectively, confirming the inhibition of acid production. Compared with the control group considered as showing 100% biofilm formation, the rate of biofilm formation was only 32% in the presence of 0.05% HT_01. HT_01 showed potent antibacterial activity against oral pathogens. HT_01 showed potent inhibitory activity against acid production and biofilm formation in S. mutans.
Source: Natural Product Sciences
Authors: So Won Kang, LIM JEONG HYEON, Sungsook Choi, Chu-Youn Song
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funder: Ministry of SMEs and Startups
Award: G7458700668
OpenAlex ID: W4415366175