Using the A-RISC index to predict IgE cross-reactivity in legume allergens: An in silico approach
Year: 2025
Type: article
Abstract: INTRODUCTION: Legumes are a common source of allergic sensitization in many regions worldwide. Structural similarity among homologous proteins can lead to IgE-mediated cross--reactivity. In this context, in silico analysis offers a valuable approach to predict potential molecular interactions among related allergens and to support the interpretation of risk in patients with multiple sensitizations. METHODS: An in silico analysis was conducted to evaluate sequence homology, structural conservation, and surface exposure of IgE epitopes across five major protein families: 11S globulins, 7S globulins, 2S albumins, nsLTPs, and PR-10. Tools included multiple sequence alignment, A-RISC index calculation, and 3D visualization with ChimeraX. RESULTS: PR-10 proteins exhibited high homology (A-RISC >0.75), suggesting a high risk of cross-reactivity. Vicilins and glycinins showed intermediate similarity (A-RISC 0.45-0.57), while nsLTPs and 2S albumins displayed low A-RISC values (<0.50), although conserved structural motifs were identified in immunologically relevant regions. CONCLUSION: This in silico approach enables early identification of cross-reactivity potential, reinforces the value of component-resolved molecular diagnostics, and contributes to improved food labeling, clinical decision-making, and nutritional safety in patients with -multiple sensitizations.
Source: Allergologia et Immunopathologia
Institutions: Universidad El Bosque, Fundación Neumológica Colombiana
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,000
Funder: Universidad El Bosque
OpenAlex ID: W4415380674