Year: 2025
Type: article
Abstract: We introduce super-resolution panoramic integration (SPI), an on-the-fly microscopy technique enabling instantaneous generation of subdiffractional images concurrently with scalable, high-throughput screening. SPI leverages multifocal optical rescaling, high-content sweeping, and synchronized line-scan readout while preserving minimal post-processing and compatibility with epi-fluorescence settings. We demonstrate SPI for various subcellular and populational morphology, function, and heterogeneity. This versatile platform offers a practical pathway toward biological insights beyond traditional optical and computational constraints.
Source: Nature Communications
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
Funders: National Science Foundation, U.S. Department of Health and Human Services, Georgia Institute of Technology, Emory University, Georgia Research Alliance, Institute for Bioengineering and Biosciences, National Institutes of Health, Parker H. Petit Institute for Bioengineering and Bioscience, National Institute of General Medical Sciences, Division of Electrical, Communications and Cyber Systems
Awards: EFRI CEE: Human cardiac opto-epigenetics with HDAC inhibitors, G2076182236, Super-Resolution Cell and Tissue Imaging via Parallel Multifocal Scanning Microscopy, G2816264552, LTREB: The Multicellularity Long Term Evolution Experiment (MuLTEE), G3824688650, Exploring Single-Molecule Biophotonics for Ultrahigh-Resolution Spatiotemporal-Multiplexed Optical Microscopy, G4711046150, NNCI: Southeastern Nanotechnology Infrastructure Corridor (SENIC), G5868592039, G5927359219, Using directed evolution to study the origins of multicellular development., G790860305, CAREER: Multiplexing Light-Field Microscopy for Cell Biological Research, G9000061502, G9000192359
OpenAlex ID: W4415403148