Work

Refined mechanism of promoter nucleosome-depleted regions resetting after replication

Year: 2025

Type: article

Abstract: Replication disrupts chromatin organization. Thus, the rapid resetting of nucleosome positioning is essential to maintain faithful gene expression. The initial step of this reconfiguration occurs at nucleosome-depleted regions (NDRs). While studies have elucidated the role of transcription factors (TFs) and chromatin remodelers (CRs) in vitro or in maintaining NDRs in vivo, none has addressed their in vivo function shortly after replication. Through purification of nascent chromatin in yeast, we dissected the choreography of events governing the proper positioning of the -1/+1 nucleosomes flanking promoter NDRs. Our findings reveal that CRs are the primary contributors of -1/+1 repositioning post-replication, with RSC (Remodeling the Structure of Chromatin) acting upstream of INO80. Surprisingly, while Reb1 and Abf1 TFs are not essential for NDR resetting, they are required for NDR maintenance via the promotion of H3 acetylations. Altogether, we propose a two-step model for NDR resetting in Saccharomyces cerevisiae: first, CRs alone reset promoter NDRs after replication, while a combination of TFs and CRs is required for subsequent maintenance.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkaf1025

Authors: Sevil Zencir, Jatinder Kaur Gill, Françoise Stutz, Françoise Stutz, Julien Soudet

Institution: University of Geneva

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,992

Funders: National Science Foundation, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, Canton de Genève, Institute of Genetics and Genomics of Geneva