Effects of resveratrol on orthodontic tooth movement in Wistar rats: a biochemical, histological, and in silico study
Year: 2025
Type: article
Abstract: BACKGROUND: Orthodontic tooth movement (OTM) is a complex biological process influenced by oxidative stress and inflammation. This study investigated the effects of resveratrol, a natural antioxidant and anti-inflammatory compound, on OTM in Wistar rats using biochemical, histological, and in silico analyses. MATERIALS AND METHODS: Thirty female Wistar rats were divided into three groups: control, OTM, and OTM + resveratrol. OTM was induced using nickel-titanium closed-coil springs. The OTM + resveratrol group received 20 mg/kg resveratrol daily for 14 days. Total oxidant status (TOS) and total antioxidant status (TAS) levels were measured. Histopathological and immunohistochemical analyses were performed to evaluate osteoblast/osteoclast activity and TNF-α expression. In silico analyses were used to identify molecular pathways influenced by resveratrol. RESULTS: Resveratrol administration significantly reduced oxidative stress by increasing TAS and decreasing TOS levels. Histological findings showed enhanced osteoblast and osteoclast activity in the OTM + resveratrol group, indicating accelerated bone remodeling. Masson's trichrome staining demonstrated improved bone matrix integrity, whereas TNF-α immunostaining revealed a decreased inflammatory response. In silico analyses identified 19 common proteins linked to TNF-α modulation by resveratrol, with key pathways involving interleukin and immune system signaling. CONCLUSIONS: Resveratrol exhibits potent antioxidant and anti-inflammatory effects, enhances bone remodeling, and reduces TNF-α-mediated inflammation in OTM. These findings suggest its potential as a therapeutic adjunct in orthodontic treatment to mitigate the adverse effects of mechanical forces on bone metabolism.
Source: Folia Morphologica
DOI: 10.5603/fm.105425
Authors: Kamile Keskin Oruç, Fırat Aşır
Institution: Dicle University
Language: English
Subfield: Orthodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4415407886
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