Work

Microfluidic engineering of pDNA nanogels in a coaxial flow reactor: process development, optimisation, scalability and in vitro performance

Year: 2025

Type: article

Abstract: ) maintained a high GFP expression, confirming functional gene delivery and process scalability. We identified critical process parameters governing nanogel properties and scalability, including minimum residence time for nanogel formation, optimal flow rate ratios, reagent feeds configuration and reactor design for large-scale implementation. This work establishes a robust and scalable microfluidic process for producing functional polymeric nanogel gene delivery vectors, demonstrating its feasibility for translation from laboratory to larger-scale manufacturing, thereby serving as a proof of concept for future industrial-scale gene therapy applications.

Source: Nanoscale Advances

DOI: 10.1039/d5na00558b