iNOME-seq: in vivo simultaneous genome-wide mapping of chromatin accessibility, nucleosome positioning, DNA-binding protein sites, and DNA methylation in Arabidopsis
Year: 2025
Type: article
Abstract: We present iNOMe-seq, a novel method for in vivo simultaneous profiling of chromatin accessibility, nucleosome occupancy, DNA-binding protein sites, and DNA methylation in living tissues. iNOMe-seq utilizes an m5 C methyltransferase to mark accessible cytosines in a GpC context, bypassing nucleosome-restricted regions. Using Arabidopsis thaliana , we demonstrate that iNOMe-seq improves chromatin accessibility quantification compared to existing methods. Furthermore, it allows for the spatial and temporal analysis of chromatin dynamics, transcription factor binding, and DNA methylation, offering insight into the role of epigenetic components in transcriptional regulation across tissues and genetic variations in natural populations.
Source: Genome biology
Institutions: University of Warwick, European Bioinformatics Institute, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Université de Montpellier, Diversité, adaptation et développement des plantes, Institut de Recherche pour le Développement, Max Planck Institute for Biology, Centre National de la Recherche Scientifique, Université Paris-Saclay, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institut des Sciences des Plantes de Paris Saclay, Princess Nourah bint Abdulrahman University, University of Essex, Queen Mary University of London, Blizard Institute
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,490
OpenAlex ID: W4415427277