Work

A Two-Stage Method for Purifying Alpha-Mangostin: Sequential Solvent Extraction and Preparative Liquid Chromatography

Year: 2025

Type: article

Abstract: This research aimed to establish the optimal conditions for the purification of alpha-mangostin from mangosteen pericarp, employing a two-stage process involving solvent extraction and liquid chromatography. Initially, a sequential solvent extraction protocol, utilizing deionized water and hexane, was implemented to effectively remove both polar and nonpolar impurities. Subsequently, alpha-mangostin was extracted using acetonitrile, and the solvent was removed through evaporation and precipitation, yielding a xanthone-rich fraction. This fraction was then subjected to quantitative liquid chromatography, wherein the influence of mobile phase flow rate, mobile phase composition, feed concentration, and column temperature on purification efficiency was systematically evaluated. The optimized purification parameters, namely a mobile phase flow rate of 4 mL/min, a mobile phase composition of 80% acetonitrile and 20% deionized water, a feed concentration of 3 mg/mL, and a column temperature of 35 °C, resulted in alpha-mangostin with a purity of 83.33%.

Source: ACS Omega

DOI: 10.1021/acsomega.5c07022

Authors: Amaraporn Kaewchada, Apivich Phumjaroen, Preuk Tangpromphan, Attasak Jaree

Institutions: King Mongkut's University of Technology North Bangkok, Kasetsart University

Language: English

Topic: Natural Compound Pharmacology Studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,935

Funder: Faculty of Engineering, Kasetsart University