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Taxonomic identification, genomic analysis, and optimized chromium(VI) bioreduction by Microbacterium triticisoli sp. nov. M28 T

Year: 2025

Type: article

Abstract: Strain M28 T was isolated from subsoil obtained from decaying wheat straw. Cells were Gram-positive, non-motile, short rod-shaped and formed yellowish colonies on lysogeny broth (LB) agar. The strain was able to grow at 0–8% (w/v) NaCl , 15–40 °C and pH 5.5–10.0. Phylogenetic analysis based on 16S rRNA gene sequences, core genes and whole-genome indicated that strain M28 T belonged to the genus Microbacterium but was distinct from all known strains in this genus. Based on phenotypic, genotypic, chemical taxono mic and phylogenetic analyses, strain M28 T is a representative of a new species of Microbacterium , which is proposed to be named Microbacterium triticisoli sp. nov., the type strain is M28 T (=CCTCC AA 2022021 T =JCM 35796 T ). Genomic analysis revealed multiple metal resistance systems, antibiotic resistance determinants and oxidative stress defense genes, explaining its exceptional environmental adaptability. Notably, the strain reduced 99% of 50 mg/L Cr(VI) within 24 h under optimized conditions (37 °C, pH 7.0, 2.5 g/L sucrose) and tolerated Cr(VI) concentrations up to 125 mg/L. This study identifies M. triticisoli as a promising agent for chromium bioremediation, providing a foundation for engineering microbial solutions to heavy metal pollution.

Source: PeerJ

DOI: 10.7717/peerj.20192

Authors: Yu Qing, Jiewei Tian, Zhenhua Ma, Miao Tang, Xiufeng Long

Institution: Guangxi University of Science and Technology

Language: English

Topic: Chromium effects and bioremediation

Subfield: Health, Toxicology and Mutagenesis

Field: Environmental Science

Domain: Physical Sciences

SDG: Clean water and sanitation

Open Access status: gold

Estimated APC paid: $1,395