Extraction of PCB18 (2,2',5-Trichlorobiphenyl) and Its Hydroxylated Metabolites from Animal Tissues v2 v1
Year: 2025
Type: preprint
Abstract: This protocol describes a method for extracting PCB18 (2,2′,5-trichlorobiphenyl) and its monohydroxylated metabolites from mouse tissues via liquid-liquid extraction followed by solid-phase extraction cleanup. The levels of PCB18 and OH-PCB18s in the tissue are subsequently measured by gas chromatography-tandem mass spectrometry.
Authors: Xueshu Li, Sean Brobston, Hans-Joachim Lehmler
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
Awards: PCB-mediated dysbiosis of the gut microbiome: A missing link in PCB-mediated neurodevelopmental disorders?, Iowa Superfund Research Program: Airborne PCBs: Sources, Exposures, Toxicities, Remediation, Environmental factors in pathobiology of dementia: the role of PCB exposure, microbiome, and tissue barrier dysfunction, Molecular and Cellular Basis of PCB Developmental Neurotoxicity
OpenAlex ID: W4415465436