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No evidence for human or rodent deltavirus replication in yeast

Year: 2025

Type: article

Abstract: Deltaviruses were recently discovered across a broad range of metazoan species. Their genome encodes a single protein and thus requires host factors for replication, which are likely evolutionarily conserved given their broad host range. Here we describe a genetic tool to unambiguously determine whether an RNA virus replicates in model yeast species. Our system involves transcriptional induction of viral RNA from an integrated cDNA template followed by its genomic excision. Testing Hepatitis D and Rodent delta viruses revealed that neither viral RNAs can replicate in yeasts, suggesting that Ascomycetes lack factors essential for their RNA-dependent amplification.

Source: PubMed

DOI: 10.17912/micropub.biology.001743

Authors: Louison Bois Naegelin, Valérie Courgnaud, Dominique Helmlinger, Karim Majzoub

Institutions: Centre National de la Recherche Scientifique, Université de Montpellier, Centre de Recherche en Biologie cellulaire de Montpellier, Institut de Génétique Moléculaire de Montpellier

Language: English

Topic: Hepatitis B Virus Studies

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W4415468922

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