Year: 2025
Type: article
Abstract: SignificanceMulti-exposure speckle imaging (MESI) is a label-free technique to visualize and measure blood flow. Accurate perfusion measurements are useful in a variety of applications, including surgery, monitoring treatment, and diagnosing various conditions.AimWe aim to demonstrate the feasibility of capturing speckle images through an optical fiber bundle for use in MESI for potential applications such as endoscopy or where free space measurements are not feasible.ApproachTo compare the accuracy of fiber bundle MESI measurements against free space MESI measurements, measurements of a tissue-simulating flow phantom and in vivo mouse cortex were acquired simultaneously through free space and an optical fiber bundle.ResultsUsing the Pearson correlation coefficient for comparing measurements, R2 values of 0.9994 and 0.9942 were calculated for low (1 to 10 μL/min) and high (10 to 100 μL/min) flow rates, respectively. For in vivo measurements, an R2 value of 0.970 was calculated for flow in 14 vessels and 5 parenchyma regions. R2 values of 0.953 and 0.906 were calculated for two vessels before, during, and after a stroke.ConclusionsMESI measurements through an optical fiber bundle show similar results to free-space MESI.
Source: Journal of Biomedical Optics
Institution: The University of Texas at Austin
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,675
Awards: G1119181033, G9000136186, G9000137498
OpenAlex ID: W4415488954