Work

Experimental study of a portable integrated optical scanning holographic microscope for 3D bioimaging

Year: 2025

Type: article

Abstract: This study develops a compact optical scanning holographic microscopy (OSHM) system integrating high - precision components into a portable cage platform (60cm × 60cm, 22.45kg). On this basis, by leveraging the structural advantages of the integrated components, the system-which combines a high numerical aperture microscope objective with dual-spherical-wave interference illumination on the sample-achieves a lateral resolution of 588 nm. Experimental verification shows that the system can achieve high-resolution 3D imaging of biological samples and demonstrates imaging results in samples such as blood cells, plant cells, and mycelium. The introduced and validated dual-spherical-wave interference encoding mechanism overcomes the limitations of conventional plane-wave-spherical-wave configurations, providing a practical example of optical scanning holography in biomedical applications.

Source: Optics Express

DOI: 10.1364/oe.576659

Authors: Jinhao Zhou, Yongwei Yao, Huanrong He, Yaping Zhang, Jilu Duan, Ting-Chung Poon

Institution: Bradley University

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funders: National Natural Science Foundation of China, Yunnan Provincial Science and Technology Department