Work

Study of optimal method of Heterodera glycines sample preparation for molecular diagnostics

Year: 2025

Type: article

Abstract: In this study, it is optimized by the methods of preparing samples for the release of genetic material (DNA) from soybean cyst-forming nematode Heterodera glycines , which is necessary for accurate identification. Various modifications of ready-made reagent kits from Russian and foreign manufacturers were tested: DNA-Extrane-2, FitoSorb, TsytoSorb, ORB-GMO-A by Sintol LLC (Russia), Proba-GS, Proba-NK by AgroDiagnostika LLC, and QIAamp DNA Mini Kit by Qiagen (Germany). By testing the PCR method In real time, the sensitivity of Heterodera glycines total DNA release was tested using a test system consisting of forward and reverse primers and a modified probe: SCNrtF (5-AAATTCCAGGCCGCTATCTC-3), SCNrtR (5CGTGGACTGAACTGAACTGGACAAAG-3), SCNrtP (5-FAM/-TGGGCTGGGTGCTTCTAGAACTTTT-/ BHQ-1/-3).

Source: RUDN Journal of Agronomy and Animal Industries

DOI: 10.22363/2312-797x-2025-20-2-274-286

Authors: Anton V. Ivanov, Galina N. Bondarenko

Institution: All-Union Scientific Research Institute of Plant Quarantine

Language: English

Topic: Nematode management and characterization studies

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

OpenAlex ID: W4415523160

FWCI:

Cites: 9

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