Work

Conserved protein Seb1 that interacts with RNA polymerase II and RNA is an antipausing transcription elongation factor

Year: 2025

Type: article

Abstract: Maturation of protein-coding precursor messenger RNA (pre-mRNA) is closely linked to RNA polymerase II (Pol II) transcription. However, the mechanistic understanding of how pre-mRNA processing is coordinated with transcription remains incomplete. Conserved proteins interacting with the C-terminal domain of the largest catalytic subunit of Pol II and nascent RNA (CID-RRM factors) were demonstrated to play a role in pre-mRNA 3'-end processing and termination of Pol II transcription. Here, we use a fully reconstituted system to demonstrate that the fission yeast CID-RRM factor Seb1 acts as a bona fide elongation factor. Our analyses show that Seb1 exhibits context-dependent regulation of Pol II pausing, capable of either promoting or inhibiting pause site entry. We propose that CID-RRM factors coordinate Pol II transcription and pre-mRNA 3'-end processing by modulating the rate of Pol II transcription.

Source: RNA

DOI: 10.1261/rna.080765.125

Authors: Krzysztof Kuś, Soren Nielsen, Nikolay Zenkin, Lidia Vasiljeva

Institutions: University of Oxford, Newcastle University

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Wellcome Trust, Biotechnology and Biological Sciences Research Council

Awards: G1495176341, Coupling of transcription with other cellular processes, Control of RNA processing during RNA polymerase II transcription, G6868616705, G7744556484