Work

Generation of an induced pluripotent stem cell line (HPCHi002-A) derived from a dilated cardiomyopathy patient harboring heterozygous mutations c.644G > T (p.Arg215Leu) in the TNNT2 gene

Year: 2025

Type: article

Abstract: The TNNT2 gene encodes the cardiac isoform of troponin T, which can regulate muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in TNNT2 have been associated with dilated cardiomyopathy. In this study, we established an induced pluripotent stem cell (iPSC) line from a dilated cardiomyopathy patient harboring the c.644G > T heterozygous mutation in TNNT2 gene using non-integrative Sendai viral reprogramming technology. This cell line exhibited typical iPSC morphology, expressed pluripotency markers, had a normal male karyotype (46XY) and maintained the ability to differentiate into the three germ layers in vitro.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2025.103861

Authors: Yongjuan Yuan, Xiaowei Li, Ningyu Ding, Jianzeng Dong, Youjian Zhang

Institutions: Henan Provincial Chest Hospital, First Affiliated Hospital of Zhengzhou University, Capital Medical University, Beijing Anzhen Hospital

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,360