Work

Protocol for lattice light-sheet time-lapse imaging of early post-implantation mouse embryos

Year: 2025

Type: article

Abstract: Lattice light-sheet microscopy offers unprecedented spatial and temporal resolution for visualizing morphogenetic and physiological processes, while minimizing photodamage. Here, we present a detailed protocol for time-lapse imaging of post-implantation mouse embryos, using lattice light-sheet microscopy. We describe steps for embryo isolation, mounting and culture, setting up of imaging parameters, and pipelines for processing the data generated in preparation for downstream analyses. This approach is also suitable for stem cell-derived embryo models, organoids, and small organ explants. For complete details on the use and execution of this protocol, please refer to Thowfeequ et al. 1 and Stower et al. 2 • Steps for dissecting post-implantation mouse embryos • Mounting post-implantation mouse embryos for lattice light-sheet imaging • Lattice light-sheet live imaging of cellular behavior in developing mouse embryos • Post-processing lattice light-sheet imaging data Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Lattice light-sheet microscopy offers unprecedented spatial and temporal resolution for visualizing morphogenetic and physiological processes, while minimizing photodamage. Here, we present a detailed protocol for time-lapse imaging of post-implantation mouse embryos, using lattice light-sheet microscopy. We describe steps for embryo isolation, mounting and culture, setting up of imaging parameters, and pipelines for processing the data generated in preparation for downstream analyses. This approach is also suitable for stem cell-derived embryo models, organoids, and small organ explants.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104153

Authors: Shifaan Thowfeequ, Helena Coker, Marco Fritzsche, Shankar Srinivas

Institutions: University of Oxford, Kennedy Institute of Rheumatology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Wellcome Trust, Biotechnology and Biological Sciences Research Council