Work

Pseudomonas aeruginosa-targeted mesoporous gold nanorod nanogenerator: NIR laser-triggered alkyl radical generation for enhanced bacterial wound treatment

Year: 2025

Type: article

Abstract: Currently, Pseudomonas aeruginosa ( P. aeruginosa ) infections pose a significant threat to public health, owing to the high morbidity, mortality and antibiotic resistance. Alkyl radical (AR)-based photodynamic therapy exhibits great potential in antibacterial applications due to its oxygen-independent advantage. Nevertheless, targeted delivery systems capable of precisely controlling AR release at bacterial infectious foci are still lacking. In the present work, a mesoporous gold nanorod (mGNR)-based multifunctional nanogenerator with P. aeruginosa -targeting and near infrared (NIR) laser-controlled AR release properties was fabricated for enhanced bacterial wound treatment. Herein, mGNR with mild-photothermal therapeutic (MPTT) effect was firstly synthesized and used as a carrier for effective loading of an AR initiator 2, 2′-azobis[2-(2-imidazolin-2-yl)propane]-dihydrochloride (AIPH). Then, cysteamine and polymer of HOOC-PEG 5000 -Mal were added in sequence to functionalize the nanosystem, obtaining nanogenerator of mGNR@AIPH@PEG-Mal. Due to the specific maleimide (Mal) and Type IV Pilus (T4P) reaction, mGNR@AIPH@PEG-Mal displayed high binding efficiency on P. aeruginosa . Upon NIR laser irradiation, mGNR@AIPH@PEG-Mal triggered AIPH cleavage to generate substantial amount of AR, which enabled high-efficient inhibition on P. aeruginosa and biofilms via the synergistic MPTT/AR antibacterial effect. Moreover, mGNR@AIPH@PEG-Mal exhibited excellent antibacterial and wound healing effect on P. aeruginosa infectious rats’ wounds. In-depth mechanism study revealed that the synergistic MPTT/AR therapeutic effect of mGNR@AIPH@PEG-Mal induced P. aeruginosa death by breaking bacterial membrane, triggering ROS boost, GSH depletion and DNA damage, as well as promoted wound healing by repolarizing M2-type macrophages and upregulating CD 31 and α-SMA expressions. In the present work, a maleimide-functionalized mesoporous gold nanorod nanogenerator with precise P. aeruginosa -targeting ability was synthesized. Upon NIR laser irradiation, the nanogenerator exhibited mild-photothermal effect (MPTT) and alkyl radical (AR)-controlled release property, which was capable of high-efficient treatment on P. aeruginosa infectious wound via the synergistic MPTT/AR effect. • A maleimide functionalized nanogenerator with precise P. aeruginosa -targeting activity was synthesized. • The nanogenerator displayed mild-photothermal effect (MPTT) and NIR-controlled alkyl radical (AR)-releasing property. • The synergistic MPTT/AR effect significantly inhibited P. aeruginosa and promoted wound healing. • The synergistic MPTT/AR effect promoted wound healing by repolarizing M2 macrophages and upregulating CD 31 and α-SMA expressions.

Source: Materials Today Bio

DOI: 10.1016/j.mtbio.2025.102457