Work

Incorporation of gas/liquid equilibration control into a perifusion system to measure tissue-specific O 2 concentration dependency of O 2 consumption rate

Year: 2025

Type: article

Abstract: Oxygen regulates a multitude of cell functions, and many diseases are linked to its delivery. In vitro experiments are critical for elucidating intracellular mechanisms. Whereas standard cell culture incubators allow exposure to varying oxygen concentrations over hours and days, systems enabling rapid and precise control of dissolved oxygen are not available. We present a system for implementing rapid changes in dissolved gas composition with a perifusion system that facilitates multiple assessments of tissue function.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00640.2025

Authors: Varun Kamat, Khang Bao, Matthew K. Grumbine, Lui Tsumura, James C. Hermanson, John Kramlich, Laura Pyle, Rayne Lim, Jennifer R. Chao, Daniel Hass, James B. Hurley, Ian R. Sweet

Institution: University of Washington

Language: English

Topic: Biomedical and Chemical Research

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funders: Research to Prevent Blindness, Leona M. and Harry B. Helmsley Charitable Trust, National Eye Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, Division of Diabetes, Endocrinology, and Metabolic Diseases

Awards: G1270133634, G1437062610, Respiration in vivo in the Retina and RPE, G3822054013, G5914818729, G6130278822, G7423674311, G8683723331, Control of Photoreceptor Metabolism, Integrated Islet Distribution Program (U24) - 2021, Diabetes Research Center, Determinants of Rod and Cone Response Characteristics

OpenAlex ID: W4415547373

FWCI: 0.869

Cites: 50

Cited by: 1

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