Year: 2025
Type: article
Abstract: ABSTRACT Recently, microbial fermentation for the synthesis of rare sugars has garnered considerable attention. d ‐Allose, a rare monosaccharide, has emerged as a significant bioproduct in the food and pharmaceutical industries due to its unique health benefits and physiological functions. However, the detection of d ‐allose in fermentation broth samples predominantly depends on complex chromatographic techniques. This study introduces a toggle‐programmed microbial fluorescent biosensor designed for the dynamic monitoring of d ‐allose. The d ‐allose‐sensing P alsR promoter, located within the alsRBACEK operon, was initially characterized and used as a core component in the biosensor. The native P T7 promoter and the P alsR promoter were then utilized for the expression of green fluorescent protein (GFP) and T7 RNA polymerase (T7 RNAP), respectively. The constitutive P pdc promoter was employed to overexpress the repressor protein AlsR in the cytoplasm, thereby ensuring effective regulation of P alsR . More importantly, a fast‐folding and fast‐degrading mGFP‐DAS replaced GFP as the reporter, facilitating a rapid response to d ‐allose during continuous microbial incubation. The results demonstrated that this novel biosensor exhibited high sensitivity to d ‐allose levels, with measurement deviation controlled within 15%. This approach offers significant advantages over traditional d ‐allose chromatography detection methods, particularly in terms of simplicity and cost‐effectiveness.
Source: Biotechnology and Bioengineering
DOI: 10.1002/bit.70088
Authors: Jun‐Jie Liu, Ling‐Jie Zheng, Fu‐Xun Yang, Hui‐Dong Zheng, Qiang Guo, Li‐Hai Fan
Institution: Fuzhou University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G1299976413, G6954352519
OpenAlex ID: W4415556574