PIONEER: A periplasmic display platform for synthetic biology-based screening of genetically encoded protein regulators
Year: 2025
Type: article
Abstract: Periplasmic display in yeast is a promising but underdeveloped method for screening and studying genetically encoded biomolecules. We present Periplasmic dIsplay Of geNetically Encoded rEgulatoRs (PIONEER), a modular platform that localizes peptides, proteins, and nanobodies to the membrane-proximal periplasmic space of Saccharomycescerevisiae, enabling direct interrogation of membrane protein function. By optimizing secretion signals (ss) and display scaffolds, PIONEER ensures stable ligand retention and robust autocrine signaling. The ability to assess peptide and protein ligand activity through signaling, rather than binding alone, marks a key advance toward function-first screening. Applied to human G protein-coupled receptors, the system enables detection of surface expression, ligand activity profiling, and classification of nanobody regulators, including antagonists and conformational stabilizers. It also distinguishes intrabodies based on their functional effects and chaperone activity. Although demonstrated with G protein-coupled receptors, PIONEER is adaptable to a wide range of membrane and soluble protein targets. As AI-driven design expands the space of candidate ligands and binders, this platform offers a scalable method for linking predicted sequences and their structures to biological function.
Source: Journal of Biological Chemistry
Authors: Jacob B. Rowe, Kyutae Lee, Daniel G. Isom
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,430
OpenAlex ID: W4416442585