Work

Evaluation of Transgenerational Gene Editing Efficiency and Inheritance of Edits Using a Split Cas9/gRNA Crossing System in Zea mays

Year: 2025

Type: article

Abstract: Transgenerational gene editing, promoter activity, editing efficiency, T-DNA copy number, CRISPR/Cas9, genetic mosaicism, mutation inheritance, single-copy events, maize CRISPR/Cas9 genome editing (GE) is used extensively in a wide variety of plant species for research and breeding.However, gene edits observed in somatic cells are not always present in the germline and inherited (Feng et al., 2014).Expression levels of the Cas9 protein is thought to be a main driver.For instance, in Arabidopsis thaliana, use of the CaMV 35S promoter to drive Cas9 expression resulted in poor edit inheritance, which was attributed to weak activity in germline cells and zygotes (Kong et al., 2021).In crops, in somatic tissue sampled from primary transformants (T0s), often more

Source: Plant Biotechnology Journal

DOI: 10.1111/pbi.70391