Molecular diagnosis of the Seville root-knot nematode Meloidogyne hispanica based on SCAR marker
Year: 2025
Type: article
Abstract: Meloidogyne hispanica has received significant attention as it poses a major threat to various crops due to its expanding geographical distribution. Consequently, there is an urgent need for specific diagnostic techniques targeting M. hispanica . However, the development of effective molecular diagnostic methods has been hindered by the lack of genomic information. To address this challenge, we developed a species-specific SCAR-PCR assay based on a RAPD marker of M. hispanica in this study. The SCAR primers were designed from the RAPD amplicon, yielding a 385-bp fragment exclusively amplified from the genome of M. hispanica . This method successfully amplified DNA from M. hispanica at all developmental stages and from infected soil samples. It exhibited high sensitivity, with detection limits equivalent to 1/1280 of a single female and 1/160 of a second-stage juvenile. This method provides a reliable tool for the specific detection of M. hispanica , enabling timely monitoring and effective containment of its spread.
Source: Phytopathology Research
Authors: Yuan Chen, Li Wang, Yanfang Sun, Tuizi Feng, Yueling Pei, Deliang Peng, Haibo Long
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
Funders: Chinese Academy of Agricultural Sciences, Chinese Academy of Tropical Agricultural Sciences, Henan Agricultural University, Yunnan Agricultural University, Guangxi University, Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences, Special Fund for Agro-scientific Research in the Public Interest, National Key Research and Development Program of China
Awards: G1320523501, G4510365578, G761378856
OpenAlex ID: W4415600284