Work

Insights of rutin-modulated aggregation of bovine hemoglobin: calorimetric, spectroscopic and microscopic arguments

Year: 2025

Type: article

Abstract: This study surveys the rutin (RTN) effect on the thermal denaturation and aggregation of bovine hemoglobin (BHb) under in vitro physiological incubation conditions using differential scanning microcalorimetry (μDSC), circular dichroism (CD), dynamic light scattering (DLS), and atomic force microscopy (AFM). Molecular docking and fractal analysis of AFM imagines support the results obtained using CD and DLS. Higher ordered fibrils occurred for long time incubated BHb, as opposed to lower ordered structures and protofibrils in the presence of RTN. The polyphenol exerts a concentration-dependent effect on the aggregation of BHb, inhibits the growth of fibril-like protein structures and promotes the formation of disordered aggregates or protofibrils. These findings may offer valuable insights in polyphenol-protein interaction, with applications in biochemistry, biophysics, and pharmaceutical research.

Source: International Journal of Biological Macromolecules

DOI: 10.1016/j.ijbiomac.2025.148574

Authors: Aurica Precupas, Mihai Anastasescu, Gianina-Elena Dobrescu, Vlad Tudor Popa

Institution: Romanian Academy

Language: English

Topic: Protein Interaction Studies and Fluorescence Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,390

Funders: Ministerul Cercetării, Inovării şi Digitalizării, Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii