Work

Pathological markers of Piezo1 at distal anastomosed ureter in predicting pyeloplasty outcomes in children

Year: 2025

Type: article

Abstract: Objective To evaluate the prognostic value of Piezo1 expression in the distal anastomosed ureter for predicting pyeloplasty outcomes. Methods Distal anastomosed ureter specimens from 28 patients diagnosed with primary ureteropelvic junction obstruction (UPJO) undergoing laparoscopic pyeloplasty were analyzed. Immunohistochemistry assessed Piezo1 expression, and Masson's trichrome staining determined the collagen-to-muscle ratio (CM ratio). Postoperative outcomes at 12 months were categorized as excellent improvement, good improvement, mild improvement, no improvement, or deterioration based on hydronephrosis severity scores (HSS). Associations between pathological markers (Piezo1 and CM ratio) and surgical outcomes were examined. Results The cohort included 28 patients (22 male, 6 female), with a median age of 64.3 months (IQR: 15.4–102.6). Compared to the unsatisfactory outcome group (no improvement/deterioration), the satisfactory group (excellent/good/mild improvement) exhibited significantly lower Piezo1 expression and CM ratio in the distal anastomosed ureter. Piezo1 expression strongly correlated with CM ratio ( r = 0.687, p < 0.0001). Piezo1 expression level threshold of <2.765% predicted satisfactory outcomes. Conclusion Distal anastomosed ureter Piezo1 expression is a promising prognostic biomarker for pyeloplasty outcomes.

Source: Frontiers in Pediatrics

DOI: 10.3389/fped.2025.1612706

Authors: Jincai Zhou, Xun Lu, Liqu Huang, Yunfei Guo

Institutions: Nantong University, Nantong University Xinglin College, Nanjing Children's Hospital, Second Affiliated Hospital of Nanjing Medical University

Language: English

Topic: Pediatric Urology and Nephrology Studies

Subfield: Pediatrics, Perinatology and Child Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801