Work

Dataset for investigating triacylglycerol accumulation in PBCV-1 infected Chlorella

Year: 2025

Type: data-paper

Abstract: Chlorella is a promising biofuel source due to its high lipid accumulation, rapid growth, and suitability for inland cultivation. However, how the Paramecium bursaria Chlorella virus 1 (PBCV-1) influences its triacylglycerol (TAG) accumulation remains underexplored. This data article provides a detailed description of the dataset generated to investigate TAG accumulation profiles in Chlorella infected with PBCV-1. The data, collected via high-resolution epifluorescence microscopy of over 4,000 cells across a full lytic cycle, includes measurements of TAG accumulation, chlorophyll fluorescence, and nuclear morphology, along with extracellular nutrient concentrations to rule out nutrient stress as a confounding factor. This dataset can be reused by researchers to develop new image analysis algorithms, train machine learning models, investigate virus-host interactions, and inform the development of more cost-effective biofuel production strategies.

Source: Data in Brief

DOI: 10.1016/j.dib.2025.112220

Authors: Amanda M. Lopez, Yoonjung Choi, Zhi Zhou

Institution: Purdue University West Lafayette

Language: English

Topic: Algal biology and biofuel production

Subfield: Renewable Energy, Sustainability and the Environment

Field: Energy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,220

Funders: U.S. Department of Agriculture, Purdue University