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Cytomorphological characteristics of the Nasopharyngeal zone Mucosa in children with Covid-19

Year: 2025
Type: article
Abstract: OBJECTIVE: Aim: To investigate cytomorphological presentations of the mucous membrane damage of the nasopharyngeal zone in Coronavirus disease in children. PATIENTS AND METHODS: Materials and Methods: A epithelium scraping from the surface of the Pharyngeal tonsils was performed with the following double fixation: the samples were fixed in a 2% solution of glutaraldehyde (25% Glutaraldehyde E. M. Grade Polyscieces Inc.) in 0.1M cacodylate buffer (Cacodylic acid Sodium salt. Fluka) at pH 7.2 for 2-2.5 hours in the cold. Washing (2 times) in cacodylate buffer was performed. The next stage was additional fixation of the samples in a 1.5% solution of OsO4 (Osmium Tetroxide. SPI - CHEM USA) in 0.1 M sodium cacodylate solution at pH 7.2 for 2 hours in the cold with subsequent washing (2 times) in buffer. Sections are made on an UMTP-6M ultramicrotome using a diamond knife (DIATOM). Sections were viewed using a TEM-100 transmission electron microscope (Ukraine). RESULTS: Results: Based on the electronic presentation, we can observe all the processes of inflammation and their consequences. We will consider the pathogenetic processes which were monitored in our studies. Inflammation consists of three main types of tissue disorders, which include: alteration, exudation, proliferation. Presentation of a section of the Palatine Tonsil epithelial cell is marked by increased focal hemorrhage and degenerative changes in the membrane of papillary structures, reactive-degenerative changes in the stroma and increased hemorrhages in the vessels, partial sclerosis and microcalcification of the Tonsil epithelial cell tissue, pronounced sclerotic changes in the epithelial cell and partial destruction of the cell membrane were also visualized, as a result of which the semiconductivity in the cell is disturbed. CONCLUSION: Conclusions: Cytological changes in the studied material of Nasopharyngeal zone in the children with Coronavirus disease can be considered as nonspecific common violation. These changes were characterized by the presence of diffuse inflammatory polymorpho-cellular infiltration, impaired hemodynamics and diffuse desquamative, dystrophic, sclerotic changes in epithelial cells, which are consistent with numerous data of scientists.
DOI: 10.36740/wlek/212502
Language: English
Field: Medicine
Open Access status: bronze
OpenAlex ID: W4415621707
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