Year: 2025
Type: article
Abstract: Human T cell development, encompassing processes in the bone marrow, thymus, and peripheral tissues, has been extensively characterized. Currently, researchers aim to recapitulate the entire T cell life trajectory ex vivo to generate optimal T cell products for therapeutic applications. Many technologies have been developed to support T cell differentiation, maturation, and activation ex vivo , initially utilizing feeder cells. However, the use of feeder cells presents challenges for clinical translation, including difficulties in standardizing production processes and risks of contamination in the final cell products. To address these issues, feeder-free technologies have been developed to facilitate human T cell differentiation from stem cells, as well as T cell maturation and activation. Here, we highlight the progress of feeder-free technologies throughout the T cell life trajectory in ex vivo settings. Additionally, we outline future perspectives on the continued development of feeder-free methodologies and their potential to improve the T cell-based treatments.
Source: Cell Biomaterials
Authors: Enbo Zhu, Zeyang Liu, Yan-Ruide Li
Institution: University of California, Los Angeles
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $9,350
OpenAlex ID: W4415629302