Comparative evaluation of extraction methods for Scorias spongiosa polysaccharides: Yield, bioactivity, and anti-aging potential
Year: 2025
Type: article
Abstract: Scorias spongiosa, a highly nutritious edible fungus native to bamboo forests, is colloquially known as “Bamboo bird's nest”. Polysaccharides are its primary bioactive ingredients, but comparative studies on their structural characteristics, in vitro antioxidant activities and anti-aging effects across different extraction methods remain scarce. In this study, Scorias spongiosa polysaccharides (SSPs) were extracted using five distinct techniques, including room-temperature water extraction (RWE, 37 °C), hot water extraction (HWE), ultrasound-assisted water extraction (UAE), ultrasound-assisted acid extraction (UAE-H), and ultrasound-assisted alkali extraction (UAE-OH). Among these techniques, UAE-OH emerged as the most efficient for SSPs preparation, yielding high polysaccharides content (25.4 ± 3.77% yield, 85.25 ± 4.13% purity) and exhibiting superior antioxidant activity. In addition, SSPs-UAE-OH possessed the robust anti-senescence effect by mitigating H2O2-induced oxidative damage, reactive oxygen species (ROS) accumulation, malondialdehyde (MDA) production and SA-β-gal expression in H9C2 cells. Structural analysis revealed that SSPs-UAE-OH primarily consists of glucose, with a molecular weight of 490 KDa, and FT-IR spectroscopy suggested its potential classification as an α/β-dextran. Furthermore, ultrasonic power was optimized to 500 W through a yield- and activity-guided approach, achieving an enhanced SSPs-UAE-OH yield (32.57 ± 1.57%) and significantly improved H9C2 cell viability (82.33 ± 3.74%). This study not only established an efficient extraction protocol for SSPs but also elucidated the structural basis of their anti-aging properties, particularly for cardiovascular disease prevention. These findings provide critical insights for developing SSPs-based functional foods.
Source: Ultrasonics Sonochemistry
Authors: Yiting Xue, Jun Zhang, Ji Yan, Junhao Qiu, Jiaxin Zeng, Yixin Li, Xiaoren Huang, Xinjue Li, Lingyuan Guo, Lingtian Wu, Qian Wang
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,710
Funders: Jiangsu Agricultural Science and Technology Innovation Fund, National Natural Science Foundation of China, China Postdoctoral Science Foundation, National University's Basic Research Foundation of China, Natural Science Research of Jiangsu Higher Education Institutions of China, Fundamental Research Funds for the Central Universities
OpenAlex ID: W4415641568