Use of patient-derived organoids for pleural mesothelioma 3-D modeling
Year: 2025
Type: article
Abstract: Using cells isolated from pleural effusion and pleural biopsy, we established an efficient 3-D culture system for generating PM and reactive mesothelial (RM) patient-derived organoids. PM-PDOs expressed a specific gene profile, preserved the histological architecture, showing markers of the parental tumor tissues and recapitulated the tumor microenvironment (e.g., macrophages and tumor lymphocytes), which is an important factor influencing responses to therapy. This approach will be useful for drug screening, contributing to a more accurate selection of therapeutic options.
Authors: Luca Volpini, Federica Monaco, Elisabetta Casalone, Elton Jalis Herman, Rebecca Filomena, Sandra Manzotti, Olga Strogovets, Gianluca Moroncini, Matteo Mozzicafreddo, Antonella Poloni, Elena Marinelli Busilacchi, Francesca Gonnelli, Francesca Barbisan, Gaia Goteri, Lina Zuccatosta, Martina Bonifazi, Giuseppe Matullo, Jiri Neuzil, Lory Santarelli, Najib M. Rahman, Kevin Blyth, Marco Tomasetti, Federico Mei
Institutions: Marche Polytechnic University, University of Turin, Azienda Ospedaliero-Universitaria delle Marche, Ospedale Antonio Cardarelli, Azienda Ospedaliera Citta' della Salute e della Scienza di Torino, Griffith University, Czech Academy of Sciences, Czech Academy of Sciences, Institute of Biotechnology, University of Oxford, Oxford University Hospitals NHS Trust, Queen Elizabeth University Hospital, University of Glasgow
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Funder: European Commission
OpenAlex ID: W4415664691