Work

Enhanced antioxidant properties and bioavailability of naringenin through EGCG-modified alfalfa ferritin nanoparticles

Year: 2025

Type: article

Abstract: Phytoferritin is widely used for encapsulating bioactive molecules due to its nanocavity and surface modification ease. This study presented a novel approach by covalently conjugating epigallocatechin gallate (EGCG) to the surface of alfalfa ferritin (AFn), forming the EGCG-modified ferritin complex (EGCG-AFn). This modification enhanced the stability of ferritin and improved the antioxidant properties of the nanoparticles. Naringenin (NAR) was then encapsulated within the EGCG-AFn cavity through temperature incubation. Fourier transform infrared spectroscopy confirmed the successful coupling of EGCG, while high-performance liquid chromatography verified NAR loading. The EGCG-AFn complex showed superior antioxidant activity compared to free EGCG and unmodified AFn. Gastrointestinal simulations indicated effective protection of NAR, and Caco-2 cell studies demonstrated enhanced cellular transport, significantly improving the bioavailability of NAR and maximizing its potential health benefits. • Stability of alfalfa ferritin was enhanced by covalent EGCG modification. • EGCG-ferritin had stronger antioxidant activity than native alfalfa ferritin. • Ferritin encapsulation enhanced NAR bioavailability and cellular transport.

Source: Journal of Agriculture and Food Research

DOI: 10.1016/j.jafr.2025.102487

Authors: Siyuan Li, Kun Yang, Dongyang Feng, Yu Zhang

Institution: Nanjing Forestry University

Language: English

Topic: Proteins in Food Systems

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Good health and well-being, Zero hunger

Open Access status: gold

Estimated APC paid: $2,530