Work

Whole genome sequence of multidrug-resistant Staphylococcus haemolyticus and Enterococcus faecalis isolates from public gymnasium equipment reveals evolving infection potential and resistance

Year: 2025

Type: article

Abstract: Whole genome sequences (WGSs) of Enterococcus faecalis S3 and Staphylococcus haemolyticus S5, isolated from gymnasium equipment in Tennessee, USA, were analyzed. The genome sizes of E. faecalis S3 and S. haemolyticus S5 were approximately 3.0 Mb and 2.5 Mb, respectively. Both isolates were found to harbor genes conferring resistance to multiple antibiotics, including tetracycline, fluoroquinolone, and macrolide. Gene cluster analysis revealed a cyclic lactone inducer cluster in both strains, which is critical for quorum sensing-mediated pathogenicity. Multilocus sequence typing (MLST) identified E. faecalis S3 as ST40 and S. haemolyticus S5 as ST52. Notably, evolutionary analysis of gene contraction and expansion in these isolates revealed an expansion of genes associated with horizontal gene transfer. This expansion likely represents an evolutionary strategy to facilitate the spread of antibiotic resistance genes to other isolates. These findings offer valuable insights into the genomic apparatus responsible for antibiotic resistance and potential transmission mechanisms in human-associated environments.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0324894

Authors: Goutam Banerjee, Pratik Banerjee

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Antimicrobial Resistance in Staphylococcus

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,382