Work

Effects of high hydrostatic pressure combined with enzymatic hydrolysis on the allergenicity, structural properties, and antigenic epitopes of soybean glycinin

Year: 2025

Type: article

Abstract: In this study, we systematically investigated the effects of high hydrostatic pressure-assisted enzymatic hydrolysis (HHP-E) on the allergenicity, structural properties, and antigenic epitopes of soybean glycinin (11S). The results showed that after 600 MPa pressure combined with enzymatic treatment (H600-E), the allergenicity of 11S decreased by 34.54%, and the allergenicity decreased by 76.68% and 71.43% after simulated digestion in adults (120 min) and infants (60 min), respectively. Spectral analysis revealed that the contents of β-sheets and random coils in the secondary structure of 11S decreased after H600-E treatment and in vitro digestion, and that the tertiary structure changed significantly. Mass spectrometry analysis revealed that after H600-E treatment, 29 dominant linear epitopes of 11S were degraded, and another six linear epitopes were further degraded after simulated digestion. These results indicated that HHP-E treatment can reduce the allergenicity of the 11S protein by altering its conformation and destroying antigenic epitopes. • HHP-E treatment significantly reduced the allergenicity of soybean glycinin. • The structures of 11S were changed after HHP-E treatment and in vitro digestion. • The antigenic epitopes were destroyed after HHP-E treatment and simulated digestion.

Source: LWT

DOI: 10.1016/j.lwt.2025.118682