Circulating metabolites associated with protection against Mycobacterium tuberculosis infection and inhibition of mycobacterial growth
Year: 2026
Type: article
Study design: Observational Study
Abstract: A significant proportion of individuals who are heavily exposed to infectious tuberculosis patients do not acquire Mycobacterium tuberculosis (Mtb) infection, as detected by an interferon gamma release assay (IGRA). We examined circulating metabolite profiles and metabolic genotypes in 199 heavily exposed IGRA-negative tuberculosis household contacts in Indonesia. Based on differentially abundant metabolites, activity of several pathways including arachidonic acid, arginine and proline, glutathione, and tryptophan metabolism, correlated with a negative IGRA at three months. SNPs near PRODH (involved in arginine and proline metabolism) were associated with circulating proline concentrations and persistently negative IGRA results, while SNPstudys near AFMID (involved in tryptophan metabolism) were associated with IGRA conversion. For further validation, plasma metabolomic profiles were correlated with mycobacterial growth inhibition by peripheral blood mononuclear cells from individuals in a low-incidence setting. Lower circulating concentrations of six metabolites, including leukotriene B4, proline, glycine, and tryptophan correlated with better growth control in non-exposed healthy individuals, as well as with stronger protection against IGRA-conversion in the Indonesian tuberculosis household contacts. Collectively, these data support the notion that circulating metabolites may impact innate host defense against Mtb infection, and that metabolic interventions may prevent tuberculosis infection and disease.
Source: Communications Biology
Authors: Todia P. Setiabudiawan, Lika Apriani, Julian Avila-Pacheco, Edwin Ardiansyah, Vinod Kumar, Bachti Alisjahbana, Ayesha J. Verrall, Andrew R. DiNardo, Agnes R. Indrati, Vera P. Mourits, L. Charlotte J. de Bree, Simone J. C. F. M. Moorlag, Clary B. Clish, Leo A. B. Joosten, Arjan van Laarhoven, Krista E. van Meijgaarden, Mihai G. Netea, Simone A. Joosten, Valerie A. C. M. Koeken, Philip C. Hill, Reinout van Crevel
Institutions: Radboud University Nijmegen, Radboud University Medical Center, Padjadjaran University, Broad Institute, Nitte University, University Medical Center Groningen, University of Groningen, University of Otago, Baylor College of Medicine, Texas Children's Hospital, Haga Hospital, Iuliu Hațieganu University of Medicine and Pharmacy, Leiden University, Leiden University Medical Center, University of Bonn, Rotterdam University of Applied Sciences, Queen Mary University of London, Blizard Institute
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $4,190
OpenAlex ID: W7161847488