Work

Effects of Charged Surface on Electrochemical Sensitivity to Protein Dimerization

Year: 2025

Type: article

Abstract: Protein dimerization is a crucial biological process in which proteins interact into homo- or heterodimers to form a functional assembly. Understanding and modulating the molecular mechanisms of protein dimerization and their function represent the cutting edge of research and provide multiple entries for biomedical applications. Label-free methods sensitive to homodimer formation are still required. Electrochemical methods are sensitive to small structural changes due to the presence of a surface and polarization to high negative/positive potentials, where partial denaturation/unfolding can appear. Since the dimeric structure is usually more flexible, the electric field effects induce more salient structural changes close to the electrode surface, accompanied by higher chronopotentiometric/voltammetric responses. Serum albumin and anterior gradient receptor-2 were studied as model homodimeric proteins.

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.5c05281

Authors: Hana Černocká, Veronika Kasalová, Eva Tihlaříková, Vilém Neděla, Roman Hrstka, Veronika Ostatná

Institutions: Czech Academy of Sciences, Czech Academy of Sciences, Institute of Biophysics, Czech Academy of Sciences, Institute of Scientific Instruments, Masaryk Memorial Cancer Institute

Language: English

Topic: Electrochemical Analysis and Applications

Subfield: Electrochemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,500