Work

Genetic and virulence factors behind the success of high-risk Pseudomonas aeruginosa clones: insights from comparative genomics and an experimental infection model

Year: 2025

Type: article

Abstract: Introduction Pseudomonas aeruginosa causes severe healthcare-associated infections. High-risk clones are defined by global dissemination and multidrug resistance, yet virulence is heterogeneous. We sought to map accessory-genome determinants associated with high-risk clones by integrating whole-genome sequencing (WGS) with a Caenorhabditis elegans infection model. Methods We analyzed 84 clinical isolates plus publicly available genomes using WGS, phylogenomics, and resistome/virulome profiling. Virulence was measured by C. elegans slow-killing (SK). A GWAS of accessory-genome subelements (AGEs) identified loci with high- (HVA) or low-virulence association (LVA). Coding sequences were annotated with Prokka and InterPro. Results Although high-risk and sporadic clones carried a similar total number of antimicrobial-resistance genes, 15/67 (22.38%) genes/variants were enriched in high-risk clones, producing class-level enrichment ( p < 0.002) for aminoglycosides, phenicols, trimethoprim, sulfonamides, and tetracyclines, but not β -lactams or fosfomycin. Many resistance determinants are recognized mobile-element cargo such as integron cassettes or plasmid/ICE-borne genes (e.g., aadA , dfrB , bla VIM-2 , crpP , cmlA , floR ), indicating a mobility-linked resistome in high-risk clones. GWAS identified 113 AGEs linked to SK virulence (42 HVA, 71 LVA). HVA regions were enriched for pyoverdine ( fpvA , pvdE , pvdD ) and LPS O-antigen ( wbpA/B/D ) loci , whereas LVA regions were enriched for ICE/conjugation/integrase motifs. cdsA and clpP were newly associated with P. aeruginosa virulence. Phenotypically, high-risk clones were more often strong biofilm producers and none were non-producers. High-risk clones were not consistently more virulent in SK, suggesting success reflects persistence traits (mobile DNA and biofilm under antibiotic pressure). Conclusion Accessory-genome GWAS revealed two risk dimensions: acute-virulence programs (HVA) versus mobility functions (LVA) favoring persistence and spread. Because SK measures acute virulence, readouts did not align with high-risk designations. Genomic reports should combine high-risk assignment with accessory-genome and effector profiling to support earlier containment and mechanism-aware, biofilm-focused care.

Source: Frontiers in Microbiology

DOI: 10.3389/fmicb.2025.1674635