Work

Rapid Glass Wool Enrichment of Glycopeptides for MALDI-MS Analysis of Immunoglobulin G Glycosylation in COVID-19 Samples

Year: 2025

Type: article

Abstract: Glycosylation is the most common protein post-translational modification, affecting protein properties and functions. Abnormal variations in glycosylation are associated with diseases, e.g., coronavirus disease COVID-19. Matrix-assisted laser desorption/ionization mass spectrometry has been widely utilized for studying protein glycosylation, after proper purification of glycopeptides or glycans using hydrophilic interaction liquid chromatography (HILIC) or laboratory-synthesized hydrophilic materials. Here, glass wool tips were developed to enrich immunoglobulin G glycopeptides and applied in the analysis of COVID-19 patient samples as a proof-of-concept. A significant decrease in galactosylation was detected in the COVID-19 patient plasma sample compared to the reference sample. The tips developed in this work provided a cheap and simple enrichment alternative to commercial HILIC tips for studying protein glycosylation.

Source: Journal of the American Society for Mass Spectrometry

DOI: 10.1021/jasms.5c00205

Authors: Yuye Zhou, Felicia Karlahag, Sophia Schedin Weiss, Sara Jamshidi, Lars Tjernberg, Åsa Emmer

Institutions: KTH Royal Institute of Technology, Karolinska Institutet

Language: English

Topic: Glycosylation and Glycoproteins Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500