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In vivo intratumoral heterogeneity in a dish: scalable forebrain organoid models of embryonal brain tumors for high‐throughput personalized drug discovery

Year: 2025

Type: article

Abstract: In vivo intratumoral heterogeneity in a dish: scalable forebrain organoid models of embryonal brain tumors for high-throughput personalized drug discoveryBrain tumors are the most prevalent solid tumors in pediatrics, with atypical teratoid and rhabdoid tumor (ATRT) and embryonal tumor with multilayered rosettes (ETMR) presenting particularly poor prognoses.The development of effective therapies is hampered by the lack of in vitro models that accurately reflect the complex tumor microenvironment (TME) and intratumoral heterogeneity observed in vivo.Traditional monolayer and tumorsphere/tumoroid cultures fail to capture these critical aspects [1], biasing them towards proliferative cell populations that respond differently to drugs than the original tumor.Likewise, in vivo models have fundamental limitations: they lack the primate-specific chromosome 19 microRNA cluster (C19MC) driver central to ETMR [2], cannot capture human-specific neurotoxicity, and are impractical for scalable drug screens [1,3].

Source: 癌症：英文版

DOI: 10.1002/cac2.70074