Figure 5 from Sucralose Consumption Ablates Cancer Immunotherapy Response through Microbiome Disruption
Year: 2025
Type: other
Abstract: Responder-derived FMT is sufficient to restore immunotherapeutic response. A, Individual tumor growth curves from Fig. 4D with FMT. B and C, C57BL/6 mice from Taconic consumed sucralose in the drinking water (0.09 mg/mL) for 2 weeks prior to tumor injection and for the duration of the experiment. Mice were injected with 2.5 × 105 MC38 cells subcutaneously and treated with 200 µg anti–PD-1 on days 9, 12, and 15. Tumor area was measured every 3 days until endpoint. Four groups all received an FMT from an anti–PD-1 responder mouse donor. The following four groups were used: antibiotic treatment pre-FMT, continued sucralose consumption (hot pink), antibiotic treatment pre-FMT, stopped sucralose consumption (light pink), no antibiotics, continued sucralose consumption (purple), no antibiotics, stopped sucralose consumption (light blue). Individual tumor growth curves (B) and a composite growth curve and OS (C) are shown. Data are a composite of three (A) or two (B and C) independent experiments with five mice per group per replicate. Error bars represent the mean ± SEM. For growth curve statistics, two-way ANOVA was used. OS statistics were calculated using a log-rank Mantel–Cox test. *, P < 0.05. abx, antibiotics; CR, complete response.
Authors: Kristin M. Morder, Madison Nguyen, Drew N. Wilfahrt, Zakaria Larbi Dahmani, Ansen B.P. Burr, Bingxian Xie, Michael Morikone, Hector Nieves-Rosado, William G. Gunn, Drew E. Hurd, Hong Wang, Steven J. Mullett, Kaitlin Bossong, Stacy L. Gelhaus, Dhivyaa Rajasundaram, Lawrence P. Kane, Greg M. Delgoffe, Jishnu Das, Diwakar Davar, Abigail E. Overacre-Delgoffe
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4415787031
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