Work

Integrated Molecular and Functional Analysis of Hop Ethanolic Extract in Caco-2 Cells: Insights into Inflammation, Barrier Function, and Transport

Year: 2025

Type: article

Abstract: Hop (Humulus lupulus L.) is a well-known medicinal and brewing plant, yet studies on the biological activity of its complete extracts remain limited. A comprehensive characterization of a full hop ethanolic extract (HLE) was conducted, integrating untargeted HPLC–MS profiling, anti-inflammatory evaluation in an inflammation-induced Caco-2 model, and transport assessment across intestinal epithelial monolayers. After ultrafiltration to remove pyrogenic components, HLE reduced IL-6 secretion in a concentration-dependent manner and decreased IL-8 levels, while mitigating IL-1β–induced barrier disruption as reflected by TEER recovery. HPLC–MS analysis of the basolateral compartment revealed selective permeability of medium-sized bitter-acid derivatives and the presence of three features not detected in the original extract, suggesting metabolic transformation during epithelial passage. Overall, the complete extract exhibited moderate but biologically relevant anti-inflammatory and barrier-protective effects in intestinal epithelial cells. The use of the whole extract, without isolating individual fractions, represents a practical and physiologically meaningful approach that may facilitate its application in the formulation of functional foods or dietary supplements.

Source: International Journal of Molecular Sciences

DOI: 10.3390/ijms262110608

Authors: Ruben Emmanuel Verhelst, Aleksandra Kruk

Institution: Medical University of Warsaw

Language: English

Topic: Hops Chemistry and Applications

Subfield: Pharmacology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,499