Chick embryo development assessment and fertility detection using pixel-wise hyperspectral image analysis and deep learning
Year: 2025
Type: article
Abstract: Accurate early detection of fertility and embryonic development during incubation is essential for enhancing hatchability efficiency in poultry production. This study employed a line-scan hyperspectral imaging (HSI) system to monitor fertility and embryonic development in white-shelled eggs. Full-transmittance hyperspectral images were acquired at the end of each of the first four incubation days from both fertile and infertile eggs. Classification based on average spectra was performed using soft independent modeling of class analogy (SIMCA), linear discriminant analysis, quadratic discriminant analysis (QDA), and artificial neural networks (ANN). Among these, ANN achieved the highest performance in embryonic stage detection (F1-score: 92.33 %), while SIMCA, ANN, QDA, and ANN provided the best fertility detection on days 1-4, respectively (F1-scores: 77.78-100 %). Pixel-wise classification, incorporating ANN, random forest, deep neural networks (DNN), and convolutional neural networks (CNN), further improved accuracy and enabled spatial visualization. The DNN achieved the highest stage discrimination (F1-score: 94.95 %), while CNN, DNN, ANN, and DNN performed best in fertility detection on days 1-3 (F1-score: 90.00 %) and day 4 (F1-score: 100.00 %), respectively. These findings demonstrate the potential of HSI combined with pixel-wise deep learning classification as a non-destructive and accurate method for real-time fertility assessment in poultry hatcheries.
Source: Poultry Science
Institutions: Isfahan University of Technology, University of Kashan
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,600
Funder: Isfahan University of Technology
OpenAlex ID: W4415816174