Year: 2025
Type: article
Study design: Observational Study
Abstract: Background: Cholangiocarcinoma (CCA) is an aggressive malignancy that poses a serious threat to long-term survival. In this study, we compared the biliary microbiota and metabolomic profiles of patients with CCA and those with choledocholithiasis to identify characteristic microbial species and metabolites associated with CCA and to explore the mechanisms linking microbial dysbiosis to CCA development. Methods: A total of 25 CCA patients and 25 choledocholithiasis patients were included in the study. Bile was collected intraoperatively and analyzed using 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC-MS) to investigate the correlation between specific microorganisms and metabolites by integrating microbiomics and metabolomics. Results: The abundance and diversity of microorganisms were similar between the two groups; however, their microbial compositions were significantly different. Microbial-metabolite interactions may contribute to CCA development through pathways such as inflammation, oxidative stress, and energy metabolism. Conclusion: These findings reveal a unique microbial community structure and metabolic profiles in CCA patients, providing potential microbial and metabolic markers for early CCA diagnosis. They also lay a theoretical foundation for the development of novel therapeutic strategies.
Source: Frontiers in Microbiology
Authors: Xiangyu Wang, Xue Liu, Wang Niu, Chenyue Guan, Chunlong Liu, Jiangtao Yu, Kun Song
Institution: Wannan Medical College
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4415826864